AbSolute® High Cap is a high performance Protein A media which has been developed for the capture of monoclonal and polyclonal antibodies from large volume fermentation feedstock. It is based on a modified silica matrix that exhibits strong mechanical, thermal and chemical stability.
Exceptional mechanical properties combined with a unique kinetic performance allow operations at higher velocities (up to 1,500cm/h, depending on column size and equipment).
The epoxy coating of AbSolute® High Cap has been optimised for chemical resistance and Non Specific Binding (NSB). Silica backbone degradation and Protein A leakage during NaOH exposure is thus prevented. Microscopic views of the particles, including a cross section and surface of the particles are shown below.
Surface of Particle
Small particle size: 35 ± 2μm
Perfect spherical shape
Narrow particle size distribution
Pore size: 100 ± 15nm
Pore volume: 1.6 – 1.8ml/g
Improves productivity by 200 – 300% compared with industry standards
Ligand density is more than 10mg/ml
Capture of monoclonal and polyclonal antibodies
AbSolute® is engineered to give the highest Dynamic Binding Capacities (DBC) at low and high linear velocities with high selectivity, which reduces resin consumption and improves productivity.